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In vitro genotoxicity of root canal sealers
V Brzovic1, I Miletic, D Zeljezic
1Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
International Endodontic Journal
|February 21, 2009
Summary
Root canal sealers showed minimal genotoxicity after 24-hour elution, indicating acceptable biocompatibility. Early exposure to some sealers, like Diaket, Hermetic, and SuperEBA, may cause DNA damage.
Area of Science:
- Endodontics
- Cytogenetics
- Materials Science
Background:
- Root canal sealers are crucial for endodontic treatment success.
- Evaluating sealer genotoxicity is vital for patient safety and biocompatibility.
- Leakage can influence the cytotoxic effects of endodontic materials.
Purpose of the Study:
- To assess the genotoxicity of various root canal sealers ex vivo.
- To determine the impact of leakage duration on sealer genotoxicity.
- To compare genotoxic effects using comet assay and chromosomal aberration analysis.
Main Methods:
- Six root canal sealers with different compositions were tested.
- Human peripheral blood lymphocytes were exposed to sealer eluates.
- Comet assay and chromosomal aberration analysis were employed to measure DNA damage.
Main Results:
- Short-term elution (1 hour) of Diaket, Hermetic, and SuperEBA showed significant genotoxic effects.
- DNA migration in the comet assay was more sensitive than chromosomal aberrations.
- No genotoxic effects were observed after 24-hour elution for any sealer.
Conclusions:
- All tested root canal sealers demonstrated acceptable biocompatibility regarding genotoxicity under the study conditions.
- Early leakage may pose a transient genotoxic risk, but this diminishes over time.
- The choice of cytogenetic assay influences the detection of genotoxic potential.
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