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Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
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Related Experiment Video

Updated: Jun 25, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

p150/glued modifies nuclear estrogen receptor function.

Soo Jung Lee1, Christina Chae, Michael M Wang

  • 1Department of Neurology, University of Michigan, Ann Arbor, MI 48109-5622, USA.

Molecular Endocrinology (Baltimore, Md.)
|February 21, 2009
PubMed
Summary
This summary is machine-generated.

The dynactin component p150/glued enhances estrogen receptor (ER) function by acting within the nucleus, not by altering ER localization. This protein influences estrogen sensitivity and gene transcription.

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Last Updated: Jun 25, 2026

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09:07

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Published on: August 13, 2019

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Endocrinology

Background:

  • Estrogen receptors (ERs) regulate gene expression by binding to DNA.
  • Cytoskeletal elements, including microtubules and dynein light chain 1, are implicated in ER scaffolding, nuclear transport, and estrogen-mediated gene regulation.

Purpose of the Study:

  • To investigate the direct role of the dynactin component p150/glued in nuclear estrogen receptor (ER) function and estrogen signaling.
  • To determine if p150/glued influences ER subcellular localization or directly modulates nuclear ER activity.

Main Methods:

  • Overexpression of p150/glued and ERalpha to assess effects on estrogen responses.
  • Microtubule disruption experiments to evaluate the impact on nuclear ERalpha levels.
  • Analysis of p150/glued localization within the nucleus.
  • Chromatin immunoprecipitation (ChIP) to detect p150/glued recruitment to the pS2 promoter.
  • siRNA-mediated knockdown of p150/glued in MCF-7 cells to assess effects on estrogen-dependent transcription.

Main Results:

  • Overexpression of p150/glued enhances estrogen responses, suggesting a role in potentiating ERalpha function.
  • Microtubule disruption does not alter nuclear ERalpha levels, indicating p150/glued's effect is independent of gross subcellular localization changes.
  • Modest amounts of p150/glued are found in the nucleus, supporting a direct nuclear role.
  • p150/glued is recruited to the pS2 promoter upon hormone stimulation.
  • Knockdown of p150/glued reduces estrogen-dependent transcription in MCF-7 cells.

Conclusions:

  • The dynactin component p150/glued directly modulates the potency of nuclear estrogen receptor (ER) function.
  • p150/glued enhances estrogen sensitivity through nuclear mechanisms, influencing estrogen-dependent gene transcription.
  • These findings reveal a novel nuclear role for p150/glued in estrogen signaling pathways.