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Optimization of production of caffeine demethylase by Pseudomonas sp. in a bioreactor
Sathyanarayana N Gummadi1, Swati Sucharita Dash, Santhosh Devarai
1Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, India. gummadi@iitm.ac.in
Abstract:
The effect of pH, aeration rate, and agitation rate on specific productivity of caffeine demethylase from Pseudomonas sp. was studied in a bioreactor. Maximum specific productivity of caffeine demethylase of 2,214 U g cell dry weight(-1) h(-1) was obtained at 0.27 vvm, 700 rpm, and pH 7.0. Under these conditions, volumetric oxygen transfer coefficient was 74.2 h(-1), indicating that caffeine demethylase production by Pseudomonas sp. was highly oxygen-dependent. Different metabolite formation at different agitation and aeration rates can be used as a strategy for recovery of pharmaceutically important metabolites from caffeine by manipulation of conditions in a bacterial culture. This is the first report on production of high levels of caffeine demethylase in bioreactors.
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