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Published on: December 8, 2023
Two-dimensional chromatography method applied to the enantiomeric determination of lansoprazole in human plasma by
Ricardo F Gomes1, Neila M Cassiano, J Pedrazzoli
1Departamento de Química, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil.
Abstract:
A two-dimensional HPLC method based on the direct injection of biological samples has been developed and validated for the determination of lansoprazole enantiomers in human plasma. The lansoprazole enantiomers were extracted from the biological matrix using an octyl restricted access media bovine serum albumin column (C8 RAM BSA) and the enantioseparation was performed on an amylose tris(3,5-dimethoxyphenylcarbamate) chiral column using acetonitrile:water (35:65 v/v) and UV detection at 285 nm. Analysis time was 25 min with no time spent on sample preparation. The method was applied to the analysis of the plasma samples obtained from nine Brazilian volunteers who received a 30 mg oral dose of racemic lansoprazole and was able to quantify the enantiomers of lansoprazole in the clinical samples analyzed.
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