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Published on: January 26, 2024
Typing of dengue viruses in clinical specimens from central Thailand
Vasant Khachonsaksumet1, Charnchudhi Chanyasanha, Dusit Sujirarat
1Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmwym@mahidol.ac.th
Abstract:
Typing of dengue viruses was done for clinical specimens from a total of 136 patients (children under 15-years-old) suspected of having dengue virus infections and who had been admitted to Pathum Thani Provincial Hospital during the period May 1999 to April 2000. Altogether, 44 strains were isolated (isolation rate: 32.4%), consisting of 18 DEN-1, 18 DEN-2, 7 DEN-3 and 1 DEN-4. The isolation rate decreased according to the number of days after the onset of disease, from day 4 to day 8.
Insights
This study identified dengue virus serotypes in 136 children, isolating 44 strains including DEN-1, DEN-2, DEN-3, and DEN-4. Dengue virus isolation rates decreased significantly after four days from disease onset.
Area of Science:
- Virology
- Infectious Diseases
- Epidemiology
Background:
- Dengue virus infections pose a significant public health threat, particularly in tropical and subtropical regions.
- Accurate identification and typing of dengue virus serotypes are crucial for effective disease surveillance and control.
Purpose of the Study:
- To determine the distribution of dengue virus serotypes in pediatric patients in Pathum Thani Province, Thailand.
- To investigate the relationship between the duration of illness and dengue virus isolation rates.
Main Methods:
- Clinical specimens were collected from 136 children suspected of dengue virus infection admitted to Pathum Thani Provincial Hospital.
- Dengue virus typing was performed on isolated strains using established laboratory methods.
- Isolation rates were analyzed based on the number of days from disease onset.
Main Results:
- A total of 44 dengue virus strains were isolated, with an overall isolation rate of 32.4%.
- The predominant serotypes identified were Dengue virus type 1 (DEN-1) and Dengue virus type 2 (DEN-2), each accounting for 18 isolates.
- Dengue virus type 3 (DEN-3) had 7 isolates, and Dengue virus type 4 (DEN-4) had 1 isolate.
- The isolation rate of dengue virus decreased significantly from day 4 to day 8 after the onset of disease.
Conclusions:
- This study highlights the circulation of multiple dengue virus serotypes in pediatric populations in the studied region.
- Early diagnosis and specimen collection within four days of symptom onset are critical for successful dengue virus isolation and typing.
