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Related Experiment Video

Updated: Jun 25, 2026

Quantification of Atherosclerosis in Mice
06:59

Quantification of Atherosclerosis in Mice

Published on: June 12, 2019

Proteomic profiling in apolipoprotein E-deficient mice during atherosclerosis progression.

Evrim Dursun1, Beste Ozben, Emanuela Monari

  • 1Medical Faculty, Department of Biochemistry, Akdeniz University, 07070 Antalya, Turkey.

Acta Histochemica
|February 24, 2009
PubMed
Summary

Researchers compared serum protein profiles in apolipoprotein E-deficient (Apo E(-/-)) mice with control mice to identify changes during atherosclerosis development. Significant differences in 107 protein clusters were found, aiding the search for early atherosclerosis biomarkers.

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Area of Science:

  • Cardiovascular Research
  • Proteomics
  • Animal Models

Background:

  • Atherosclerosis is a leading cause of global mortality.
  • Apolipoprotein E-deficient (Apo E(-/-)) mice are a valuable model for rapid atherosclerosis studies.
  • Understanding serum protein changes during disease progression is crucial.

Purpose of the Study:

  • To compare serum protein profiles in Apo E(-/-) mice with control C57BL/6 mice during atherosclerosis development.
  • To identify potential protein biomarkers for early atherosclerosis detection.

Main Methods:

  • Serum proteomic analysis using surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS).
  • Utilized three chip types: CM-10 (cation exchange), H50 (reversed-phase), and IMAC-30 (affinity capture).

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Last Updated: Jun 25, 2026

Quantification of Atherosclerosis in Mice
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Published on: June 12, 2019

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
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Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

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  • Statistical analysis of protein profiles using ProteinChip data manager 3.0.

    • Main Results:

    • Apo E(-/-) mice exhibited early atherosclerotic lesions by 20 weeks of age.
    • Significant differences (p<0.05) were observed in 107 out of 742 serum protein/peptide clusters between atherosclerotic and control groups.
    • Distinct protein profiles indicate molecular changes associated with atherosclerosis progression.

    Conclusions:

    • Serum protein profiles change significantly during atherosclerosis development in Apo E(-/-) mice.
    • These findings support the potential discovery of novel protein biomarkers for early atherosclerosis diagnosis.
    • The study highlights the utility of proteomic analysis in understanding complex disease mechanisms.