Related Experiment Video
Updated: Jun 25, 2026
11:30
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
Published on: January 20, 2017
Yeast two-hybrid detection of integrase-host factor interactions
Jean-Christophe Rain1, Alexandra Cribier, Annabelle Gérard
1Hybrigenics S.A., 3-5 Impasse Reille, Paris 75014, France.
Methods (San Diego, Calif.)
|February 24, 2009
Summary
We identified key cellular binding partners of HIV-1 integrase using yeast two-hybrid screening. Human lens epithelium-derived growth factor (LEDGF/p75) was confirmed to be crucial for HIV-1 integration.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- HIV-1 integrase is essential for viral replication.
- Identifying host factors interacting with integrase is crucial for understanding viral mechanisms and developing therapeutics.
Purpose of the Study:
- To identify cellular binding partners of HIV-1 integrase.
- To validate the role of identified partners in the HIV-1 replication cycle.
Main Methods:
- Systematic yeast two-hybrid screening with complex cDNA libraries.
- Efficient mating procedures for genetic saturation of positive clones.
- Rebound screens and loss-of-affinity mutants to assess interaction specificity and functional relevance.
Main Results:
- Identified several cellular binding partners of HIV-1 integrase, including LEDGF/p75, TNPO3, VBP1, and SNF5.
- Validated the critical role of human lens epithelium-derived growth factor (LEDGF/p75) in the HIV-1 integration step.
- Determined interaction regions within proteins.
Conclusions:
- Developed robust methods for identifying protein-protein interactions relevant to HIV-1.
- Confirmed LEDGF/p75 as a key host factor for HIV-1 integration.
- Identified novel potential therapeutic targets for HIV-1 infection.
