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Separation of the molecular forms of the insulin-like growth factor (IGF)-Binding proteins by affinity chromatography

Olgica Nedić1, Romana Masnikosa

  • 1INEP-Institute for the Application of Nuclear Energy, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|February 24, 2009
PubMed

Association of IGFBP-1, IGFBP-2 and IGFBP-3 with other proteins in human serum and placental cell membranes was investigated using affinity chromatography matrix with immobilized antibodies. Circulating IGFBP-1 was found to be predominantly bound to alpha(2)-macroglobulin and not in the binary complex with its ligand, IGFBP-2 complexes and/or polymers were detected, which was not acknowledged before, and IGFBP-3 molecular forms were differentiated into those that form binary/ternary complexes and those that form stable associations with other serum proteins. As for placental membranes, both IGFBP-1 dimers and high molecular mass IGFBP-1 associations, most probably with alpha(2)-macroglobulin, were recognized and resolved.

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