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Related Concept Videos

Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

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Related Experiment Video

Updated: Jun 25, 2026

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
07:20

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

Stabilized beta-catenin in thymic epithelial cells blocks thymus development and function.

Saulius Zuklys1, Jason Gill, Marcel P Keller

  • 1Department of Clinical-Biological Sciences, Laboratory of Pediatric Immunology, University of Basel, and Basel University Children's Hospital, Basel, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|February 24, 2009
PubMed
Summary
This summary is machine-generated.

Precise Wnt signaling regulation in thymic epithelial cells (TECs) is crucial for thymus development. Aberrant Wnt activation disrupts the thymic microenvironment, leading to severe immunodeficiency.

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Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
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Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

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Last Updated: Jun 25, 2026

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
07:20

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • Thymic T cell development relies on thymic epithelial cells (TECs).
  • Molecular mechanisms governing TEC differentiation and maintenance are poorly understood.
  • The role of Wnt signaling in thymus organogenesis requires elucidation.

Purpose of the Study:

  • To investigate the role of canonical Wnt signaling in TECs during thymus development.
  • To determine the impact of Wnt signaling dysregulation on thymic microenvironment formation and T cell differentiation.

Main Methods:

  • Utilized a mouse model with a stabilizing mutation of beta-catenin specifically in TECs.
  • Analyzed the effects of activated Wnt signaling on thymic epithelial cell fate commitment.
  • Assessed thymic microenvironment composition, hematopoietic precursor immigration, and T cell development.

Main Results:

  • Activation of canonical Wnt signaling in TECs altered endodermal epithelial commitment, preventing proper thymic microenvironment formation.
  • Wnt pathway dysregulation restricted hematopoietic precursor immigration into the thymus.
  • Intrathymic T cell differentiation was arrested at an early stage, resulting in severe immunodeficiency.

Conclusions:

  • Canonical Wnt signaling requires precise regulation within thymic epithelia for normal thymus development.
  • Disruption of Wnt signaling in TECs severely impairs thymus organogenesis and immune function.
  • This study highlights Wnt signaling as a critical regulator of thymic epithelial cell biology and T cell development.