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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Cell-based olfactory biosensor using microfabricated planar electrode.

Sang Hun Lee1, Sang Beom Jun, Hwi Jin Ko

  • 1School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.

Biosensors & Bioelectronics
|February 25, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel olfactory biosensor using microfabricated electrodes to detect odorant-receptor binding. This system quantifies cellular electrical responses, enabling the screening of ligands for olfactory receptors.

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Area of Science:

  • Neuroscience
  • Biotechnology
  • Sensor Technology

Background:

  • Olfactory perception begins with odorant molecules binding to specific olfactory receptors.
  • This binding triggers signal transduction, leading to changes in olfactory sensory neuron membrane potential.
  • Measuring these electrical changes is crucial for understanding olfactory mechanisms.

Purpose of the Study:

  • To develop a cell-based biosensor for detecting odorant-receptor interactions.
  • To quantitatively measure the electrical responses generated by olfactory signaling.
  • To enable screening of ligands for olfactory receptors, including orphan receptors.

Main Methods:

  • Utilized human embryonic kidney (HEK)-293 cells expressing the olfactory receptor I7.
  • Transfected cells with gustatory cyclic nucleotide gated (CNG) channels to amplify membrane potential changes.
  • Employed a microfabricated planar electrode to measure extracellular field potentials.
  • Stimulated cells with a specific odorant and measured intracellular Ca(2+) influx.

Main Results:

  • Successfully measured extracellular field potentials generated by Ca(2+) influx.
  • Detected an approximate 10 mV extracellular field potential.
  • Demonstrated quantitative measurement of intracellular Ca(2+) influx via planar electrode detection.
  • Validated the biosensor's ability to detect odorant-receptor binding events.

Conclusions:

  • A functional cell-based olfactory biosensor was successfully developed.
  • The microfabricated planar electrode provides a sensitive detection method for olfactory signaling.
  • This biosensor is valuable for high-throughput screening of ligands targeting olfactory receptors.