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Published on: July 8, 2011
Investigation of Yersinia pestis in Xenopsylla astia
Hassan Nekouie1, Mohamad Reza Razavi, Gholamhosein Seyedipoor
1Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran. H_Nekouie@yahoo.com
Abstract:
In this study, at the Department of Parasitology in the Pasteur Institute of Iran, Xenopsylla were allowed to feed on mice infected with Yersinia pestis. After 24-48 hours, they were killed by ether and kept in alcohol (70%) for 20 minutes. They were then examined for pathological signs and bacilli in different tissues and organs, longitudinally and cross-sectionally. The samples were studied using conjugated antibody and fluorescence microscopy. The results of this study revealed that the bacilli are abundant in the proventriculus after 6 hours, but it was found in other organs, rarely.
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