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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Kinome profiling using peptide arrays in eukaryotic cells.

Kaushal Parikh1, Maikel P Peppelenbosch, Tita Ritsema

  • 1Department of Cell Biology, University Medical Center Groningen, Groningen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2009
PubMed
Summary

Kinome analysis using peptide arrays reveals crucial signaling pathways controlling eukaryotic cell fate. This comprehensive approach identifies key proteins involved in cell cycle, survival, and differentiation across diverse eukaryotic systems.

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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07:44

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Published on: November 18, 2014

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Systems Biology

Background:

  • Advanced technologies like mass spectrometry have mapped eukaryotic transcriptomes and proteomes.
  • Understanding signaling pathways is critical for elucidating cell fate determination.
  • Traditional methods for studying signaling pathways are often limited to single genes or pathways.

Purpose of the Study:

  • To define proteins involved in signaling pathways that control cell fate.
  • To develop a comprehensive approach for revealing active signaling pathways in nucleated cells.
  • To describe kinome analysis techniques and their application in understanding eukaryotic cell function.

Main Methods:

  • Application of kinome analysis techniques utilizing peptide arrays.
  • Analysis of signaling pathways across various eukaryotic organisms (animal, plant, fungal).
  • Standardized procedures and analysis methods applicable regardless of the biological source.

Main Results:

  • Kinome analysis successfully identifies signaling pathways without a priori assumptions.
  • Demonstrated substantial success across diverse eukaryotic life branches.
  • Generated descriptions of cellular signaling for a broad range of organisms.

Conclusions:

  • Kinome analysis provides a powerful, comprehensive method for studying eukaryotic cell signaling.
  • This approach is essential for understanding the molecular mechanisms governing cell fate.
  • Identifying critical signaling pathways enhances our understanding of eukaryotic cell function.