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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

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Related Experiment Video

Updated: Jun 25, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

Tissue analysis with high-resolution imaging mass spectrometry.

A F Maarten Altelaar1, Ron M A Heeren

  • 1FOM Institute for Atomic and Molecular Physics FOM-AMOLF, Amsterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2009
PubMed
Summary
This summary is machine-generated.

Molecular imaging mass spectrometry precisely locates biomolecules and drugs in tissues. Technological advancements overcome previous limitations in speed, resolution, and sensitivity.

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Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

Area of Science:

  • Biomedical imaging
  • Analytical chemistry
  • Molecular pathology

Background:

  • Molecular mass spectrometry provides spatial information on biomolecules within tissues.
  • Understanding molecular distribution is crucial for disease diagnosis and drug development.

Purpose of the Study:

  • To highlight the advancements in imaging mass spectrometry.
  • To emphasize its expanding capabilities in molecular analysis of tissues.

Main Methods:

  • Utilizing advanced molecular mass spectrometric imaging techniques.
  • Analyzing tissue sections for biomolecule and drug localization.

Main Results:

  • Precise localization of biomolecules, drugs, and metabolites achieved.
  • Biomolecular aberrations targeted and identified.
  • Overcoming previous limitations in speed, resolution, and sensitivity.

Conclusions:

  • Imaging mass spectrometry is a powerful tool for molecular tissue analysis.
  • Technological progress has removed previous application barriers.
  • Enables deeper insights into biological processes and disease mechanisms.