Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting the Rate Structure of an Evolved Metabolic Network.

Metabolites·2025
Same author

Using Virtual Patients to Support Empathy Training in Health Care Education: An Exploratory Study.

Simulation in healthcare : journal of the Society for Simulation in Healthcare·2023
Same author

Toward advanced ionic liquids. Polar, enzyme-friendly solvents for biocatalysis.

Biotechnology and bioprocess engineering : BBE·2021
Same author

Using Metabolomics to Identify Cell Line-Independent Indicators of Growth Inhibition for Chinese Hamster Ovary Cell-based Bioprocesses.

Metabolites·2020
Same author

Multi-Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing.

Biotechnology journal·2020
Same author

Multi-Omics Study on the Impact of Cysteine Feed Level on Cell Viability and mAb Production in a CHO Bioprocess.

Biotechnology journal·2018

Related Experiment Video

Updated: Jun 25, 2026

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
06:03

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Rapid strain improvement through optimized evolution in the cytostat.

Alan Gilbert1, Dipen P Sangurdekar, Friedrich Srienc

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, 55455-0312, USA.

Biotechnology and Bioengineering
|February 26, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed acetate-tolerant Saccharomyces cerevisiae strains for industrial applications. These engineered yeast strains exhibit improved growth and ethanol production in the presence of acetate inhibitors.

More Related Videos

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
08:11

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

Published on: June 14, 2024

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
07:26

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
06:03

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Published on: September 20, 2016

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
08:11

Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

Published on: June 14, 2024

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
07:26

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

Area of Science:

  • Biotechnology
  • Microbial Engineering
  • Industrial Microbiology

Background:

  • Acetate in lignocellulosic hydrolysates inhibits yeast growth, hindering industrial biofuel production.
  • Saccharomyces cerevisiae exhibits reduced growth rate, G1 cell cycle arrest, and increased cell size under acetate stress.
  • Development of robust yeast strains is crucial for efficient utilization of lignocellulosic feedstocks.

Purpose of the Study:

  • To isolate and characterize acetate-tolerant Saccharomyces cerevisiae mutants.
  • To investigate the genetic basis of acetate tolerance in yeast.
  • To evaluate the performance of engineered strains in ethanol production.

Main Methods:

  • Utilized cytostat cultivation technology for rapid enrichment of acetate-resistant mutants.
  • Assessed mutant growth rates under high acetate, high ethanol, and high temperature conditions.
  • Employed whole genome microarray analysis to identify gene amplifications in tolerant mutants.
  • Conducted genetic analysis, including gene overexpression and diploid crosses, to confirm gene function.

Main Results:

  • Isolated multiple acetate-resistant Saccharomyces cerevisiae mutants within 5 days.
  • Mutant strains demonstrated enhanced specific growth rates and improved ethanol production rates and titers.
  • Identified gene amplifications, including LPP1 and ENA genes (ENA1, ENA2, ENA5), associated with acetate tolerance.
  • Gene dosage of ENA genes positively correlated with acetate tolerance in diploid crosses.

Conclusions:

  • Acetate tolerance in Saccharomyces cerevisiae can be rapidly achieved through directed evolution.
  • Amplification of LPP1 and ENA genes confers significant acetate tolerance and improves ethanol production.
  • Engineered yeast strains hold promise for industrial processes utilizing lignocellulosic hydrolysates.