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Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
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Published on: December 20, 2016

A new method for using 18O to trace ozone deposition.

Jens-Arne Subke1, Sylvia Toet, David D'Haese

  • 1Stockholm Environment Institute, University of York, York YO10 5DD, UK. js51@york.ac.uk

Rapid Communications in Mass Spectrometry : RCM
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PubMed
Summary

This study introduces a new apparatus for safely generating and using isotopically labeled ozone ((18)O(3)) in plant and soil deposition studies. The method enables precise tracking of ozone uptake, overcoming previous technical challenges.

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Area of Science:

  • Environmental Science
  • Atmospheric Chemistry
  • Isotope Geochemistry

Background:

  • Isotopically labeled ozone ((18)O(3)) is valuable for tracing ozone deposition to ecosystems.
  • Previous technical difficulties in producing and handling (18)O(3) have limited its use.
  • Safe and efficient methods are needed for (18)O(3) purification, storage, and release in fumigation experiments.

Purpose of the Study:

  • To develop and present a simple apparatus for the safe generation, purification, storage, and release of isotopically enriched ozone ((18)O(3)).
  • To demonstrate the feasibility of using (18)O(3) as an isotopic tracer for studying ozone deposition in soil.
  • To adapt the apparatus for various fumigation chamber scales and durations.

Main Methods:

  • Development of a novel apparatus for controlled generation and purification of (18)O(3).
  • Safe storage and release of purified (18)O(3) for fumigation experiments.
  • Exposure of dry soil to (18)O(3) at 100 nmol mol(-1) for 1-11 hours.
  • Analysis of (18)O tracer accumulation in soil using gas chromatography/isotope ratio mass spectrometry (GC/IRMS).

Main Results:

  • The apparatus allows for safe production and handling of (18)O(3), with approximately 50% of released (18)O as O(3) and the remainder as (18)O(2).
  • Concurrent release of (18)O(2) at 100 nmol mol(-1) had no detectable impact on the larger atmospheric oxygen pool.
  • A linear increase in the (18)O/(16)O isotope ratio was observed in soil samples over time, with significant detection after 1 hour of exposure.
  • The system is adaptable for flow rates up to 1 m(3) min(-1) and durations up to 12 hours, with modifications for larger scale applications.

Conclusions:

  • The developed apparatus provides a feasible and safe method for utilizing isotopically labeled ozone ((18)O(3)) in environmental research.
  • This technique enables accurate measurement of ozone deposition to soil and potentially other surfaces.
  • The method overcomes previous technical barriers, opening new avenues for isotopic tracing in atmospheric chemistry and ecosystem studies.