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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Related Experiment Video

Updated: Jun 25, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Methylated DNA immunoprecipitation.

Kelsie L Thu1, Emily A Vucic, Jennifer Y Kennett

  • 1Department of Cancer Genetics and Developmental Biology, BC Cancer Research Centre, Canada.

Journal of Visualized Experiments : Jove
|February 26, 2009
PubMed
Summary
This summary is machine-generated.

Methylated DNA immunoprecipitation (MeDIP) enriches methylated DNA for epigenetic studies. This method isolates methylated DNA efficiently, aiding in profiling DNA methylation patterns crucial for understanding gene expression and disease development.

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Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation is crucial for gene expression, development, and disease.
  • Accurate identification of DNA methylation patterns is essential for epigenetic research.
  • Distinguishing methylated from non-methylated DNA is a key challenge.

Purpose of the Study:

  • To describe the Methylated DNA Immunoprecipitation (MeDIP) technique.
  • To highlight MeDIP's efficiency in enriching methylated DNA.
  • To outline the applications of MeDIP in various genomic studies.

Main Methods:

  • DNA fragmentation via sonication (300-1000 bp).
  • Immunoprecipitation using anti-5'mC antibody to capture methylated DNA.
  • Magnetic bead separation, washes, Proteinase K digestion, and phenol:chloroform extraction for DNA purification.

Main Results:

  • Successful enrichment of methylated DNA from a small sample size (200 ng).
  • Validation of MeDIP efficiency using PCR analysis.
  • Purified methylated DNA suitable for locus-specific and genome-wide studies.

Conclusions:

  • MeDIP is an effective method for isolating methylated DNA.
  • MeDIP facilitates methylation profiling for epigenetic and disease-related research.
  • MeDIP aids in discovering epigenetic targets for therapeutic and prognostic tools, especially in cancer.