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Published on: July 17, 2021
[A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles]
1Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400042, China. fy777777@126.com
Objective:
To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.
Methods:
Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis.
Results:
There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05).
Conclusion:
It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.
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