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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Updated: Jun 25, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Replication timing as an epigenetic mark.

Ichiro Hiratani1, David M Gilbert

  • 1Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.

Epigenetics
|February 27, 2009
PubMed
Summary
This summary is machine-generated.

Replication timing, a key epigenetic signature, changes extensively during cell differentiation. These changes correlate with gene activity and nuclear organization, defining cell states.

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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Area of Science:

  • Epigenetics
  • Genomics
  • Cell Biology

Background:

  • Replication timing is linked to chromatin accessibility but often overlooked in epigenetic studies.
  • Previous research suggested minimal replication timing changes during development, complicating its role.

Purpose of the Study:

  • To investigate genome-wide replication timing dynamics during cellular differentiation.
  • To explore the relationship between replication timing, transcriptional activity, and subnuclear organization.

Main Methods:

  • Genome-wide analysis of DNA replication timing.
  • Assessment of transcriptional activity changes.
  • Evaluation of subnuclear organization alterations.

Main Results:

  • Extensive changes in replication timing were observed during differentiation.
  • Replication timing shifts strongly correlated with altered gene expression.
  • Replication timing domains emerged as characteristic of specific cell differentiation states.

Conclusions:

  • Replication timing is a dynamic epigenetic mark crucial for cell differentiation.
  • Temporally coordinated replication domains represent functional units of chromosome structure.
  • Replication timing serves as a distinct epigenetic signature of cellular identity.