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Related Experiment Video

Updated: Jun 25, 2026

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis
04:37

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Published on: April 25, 2025

Mast cells decrease renal fibrosis in unilateral ureteral obstruction.

Duk Hoon Kim1, Sang-Ok Moon, Yu Jin Jung

  • 1Renal Regeneration Laboratory and Department of Internal Medicine, Chonbuk National University Medical School, Jeonju, South Korea.

Kidney International
|February 27, 2009
PubMed
Summary

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Mast cells protect kidneys from fibrosis by reducing inflammation and inhibiting TGF-β1-driven epithelial-to-mesenchymal transitions. Mast cell deficiency exacerbates renal damage and fibrosis in a mouse model.

Area of Science:

  • Immunology
  • Nephrology
  • Pathology

Background:

  • Mast cells play a complex role in human diseases, influencing inflammation and tissue repair.
  • Conflicting reports exist regarding mast cell involvement in the pathogenesis of kidney diseases.

Purpose of the Study:

  • To investigate the role of mast cells in renal fibrosis using a unilateral ureteral obstruction model.
  • To determine if mast cells protect against kidney damage and fibrosis.

Main Methods:

  • Utilized genetically mast cell-deficient Kit(W)/Kit(W-v) mice, wild-type mice, and adoptively reconstituted mice.
  • Induced unilateral ureteral obstruction to model renal fibrosis.
  • Assessed renal tubular damage, fibrosis, inflammatory cell infiltration (macrophages, T cells), and epithelial-mesenchymal transition (EMT) markers.

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An Immature Murine Model of Reversible Unilateral Ureteral Obstruction
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An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

Published on: April 4, 2025

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Last Updated: Jun 25, 2026

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis
04:37

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Published on: April 25, 2025

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction
06:37

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

Published on: April 4, 2025

Main Results:

  • Mast cell-deficient mice exhibited increased renal tubular damage, stromal fibrosis, and infiltration of macrophages and T cells.
  • Higher levels of profibrotic transforming growth factor-beta1 (TGF-β1) were observed in obstructed kidneys of mast cell-deficient mice.
  • Mast cell deficiency attenuated indicators of EMT, including alpha-smooth muscle actin expression and E-cadherin expression.

Conclusions:

  • Mast cells protect the kidney against fibrosis.
  • This protection is mediated by modulating inflammatory cell infiltration and TGF-β1-driven EMT.