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Related Experiment Video

Updated: Jun 25, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

A microwell array platform for picoliter membrane protein assays.

Andreas Binkert1, Philipp Studer, Janos Vörös

  • 1ETH Zurich, Institute for Biomedical Engineering, Laboratory of Biosensors and Bioelectronics, Zurich, Switzerland.

Small (Weinheim an Der Bergstrasse, Germany)
|February 27, 2009
PubMed
Summary

A new microwell chip enables sensitive protein binding detection in picoliter volumes. This high-density array allows for multiplexed immunoassays with preserved protein activity, advancing diagnostics.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Protein binding detection is crucial for diagnostics and research.
  • Existing methods often require larger sample volumes and can impact protein activity.
  • Multiplexed assays are needed for high-throughput analysis.

Purpose of the Study:

  • To develop a novel microwell chip for sensitive protein binding detection in picoliter volumes.
  • To demonstrate the capability of the chip for multiplexed immunoassays.
  • To showcase the preservation of protein activity in the microwell environment.

Main Methods:

  • Fabrication of a polydimethylsiloxane (PDMS) microwell array on a planar optical waveguide.
  • Development of a liquid handling system using a contact-mode pin and ring spotter for picoliter dispensing and rinsing.
  • Utilizing a high-sensitivity fluorescence readout system for signal detection.
  • Demonstration using annexin V binding to phosphatidylserine (PS) in lipid bilayers.

Main Results:

  • Achieved an array density of over 1000 wells cm(-2).
  • Successfully performed immunoassays with picoliter volumes, maintaining protein activity.
  • Demonstrated sensitive detection of annexin V binding to PS, showing calcium dependence.
  • Validated the system's ability to distinguish between wells with and without PS.

Conclusions:

  • The novel microwell chip enables highly sensitive, multiplexed protein binding detection in picoliter volumes.
  • The system preserves protein activity, allowing for assays in a native-like environment.
  • This technology holds promise for advancing diagnostic and research applications requiring high-throughput analysis.