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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Related Experiment Video

Updated: Jun 25, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Bacterial glycoprofiling by using random sequence peptide microarrays.

Carlos Morales Betanzos1, Maria J Gonzalez-Moa, Kathryn W Boltz

  • 1Center for Innovations in Medicine, The Biodesign Institute, Arizona State University, 1001 S. McAllister Avenue, Tempe, AZ 85287, USA.

Chembiochem : a European Journal of Chemical Biology
|February 27, 2009
PubMed
Summary

A new high-throughput platform identifies peptides binding bacterial lipopolysaccharides (LPS). This tool rapidly differentiates bacterial glycans and yields potential antimicrobial peptides.

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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

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Last Updated: Jun 25, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microbiology

Background:

  • Current glyco-analysis methods are insufficient for growing demands.
  • High-throughput (HTP) tools are crucial for advancing glyco-analysis.
  • Bacterial lipopolysaccharides (LPS) are key targets for diagnostics and therapeutics.

Purpose of the Study:

  • To develop a novel HTP screening platform for identifying peptides that bind bacterial O-antigenic glycans.
  • To discover peptides with specific binding affinities to different bacterial LPS serotypes.
  • To explore the potential of identified peptides as antiendotoxic and antimicrobial agents.

Main Methods:

  • Development of a HTP screening platform utilizing peptide microarrays and luminescent LPS-quantum dot micelles.
  • Screening of random sequence peptide libraries against E. coli and P. aeruginosa LPS.
  • Characterization of peptide-LPS interactions using flow cytometry and isothermal titration calorimetry (ITC).

Main Results:

  • Identified specific peptides binding to E. coli and P. aeruginosa LPS.
  • Peptides binding E. coli LPS were rich in aromatic and cationic amino acids and inhibited bacterial growth.
  • ITC revealed a K(d) of 1.75 µM for some peptide-LPS interactions in solution.
  • Peptide selections against P. aeruginosa LPS showed enrichment in hydrogen-bond forming amino acids.

Conclusions:

  • The developed HTP platform enables rapid differentiation of bacterial glycans.
  • Discovered peptides show potential as antiendotoxic and antimicrobial lead compounds.
  • Identified peptides can serve as diagnostic and affinity reagents on-array or on-bead.