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Electron microscopic demonstration of Romhányi's aldehyde-bisulphite-toluidine blue (ABT)-topo-optical staining
1Department of Forensic Medicine, Semmelweis Medical University, H-1091 Budapest, Ullöi u 93, Budapest, Hungary. sotpet@igaz.sote.hu
Abstract:
Romhányi's aldehyde-bisulphite-toluidine blue (ABT) topo-optical reaction was checked electron microscopically. The reaction product was found localized on the sarcolemma membrane and intercalated disc. The findings suggest that the topo-optical ABT-reaction is suitable for molecular analysis. In addition to confirming the electron microscopic suitability of the ABT-reaction it has also offered ultrastructural evidence for the reliability of the topo-optical method.
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