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Procedures for radioimmunoassay of the mouse mammary tumor virus
Cancer Research
|May 1, 1977
Abstract:
A procedure for radioimmunoassay of the major glycoprotein antigen derived from murine mammary tumor virus is described. The assay is sensitive to 0.05 ng of antigen and is highly reproducible. The antigen, gp55, has been found to be group specific and will detect viruses in 13 separate mouse strains, as wel .l as from continuous cell lines. Factors affecting the assay have been examined.
Insights
A new radioimmunoassay accurately detects gp55, a key antigen from mouse mammary tumor virus, across various mouse strains and cell lines. This sensitive and reproducible assay aids in understanding virus-related diseases.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Murine mammary tumor virus (MMTV) is a significant etiological agent in mouse mammary carcinogenesis.
- Characterization of MMTV antigens is crucial for understanding viral pathogenesis and developing diagnostic tools.
Purpose of the Study:
- To describe a sensitive and reproducible radioimmunoassay for the major glycoprotein antigen (gp55) of MMTV.
- To evaluate the specificity and applicability of the assay across different mouse models.
Main Methods:
- Development and validation of a radioimmunoassay procedure.
- Quantification of MMTV gp55 antigen using the developed assay.
- Testing the assay's performance with samples from various mouse strains and cell lines.
Main Results:
- The radioimmunoassay is highly sensitive, detecting as little as 0.05 ng of gp55 antigen.
- The assay demonstrates high reproducibility.
- gp55 antigen was detected across 13 different mouse strains and in continuous cell lines, indicating group specificity.
Conclusions:
- The described radioimmunoassay provides a reliable method for detecting MMTV gp55.
- This assay is valuable for research on MMTV, including epidemiological studies and diagnostics.
- The group-specific nature of gp55 facilitates broad application in mouse models.