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Updated: Jun 25, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
The first complete genome sequence of a chicken group A rotavirus indicates independent evolution of mammalian and
Eva Trojnar1, Peter Otto, Reimar Johne
1Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
Abstract:
Group A rotaviruses are a leading cause of gastroenteritis in humans and animals. Transmission between mammalian species and humans has been demonstrated repeatedly. Here, the first entire genome sequence (19,064 bp) of a chicken rotavirus, strain Ch-02V0002G3, is presented. A low degree of nucleotide sequence identity with the mammalian group A rotaviruses is evident for all 11 genome segments, whereas a closer relationship to available rotavirus sequences from avian species has been determined. According to a novel rotavirus classification system, new genotypes were proposed and ratified by the Rotavirus Classification Working Group for eight of the Ch-02V0002G3 genome segments, resulting in the genotype constellation G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8. Due to the low percentages of genome sequence identity, the different genome segment sizes and the marked sequence differences of non-structural proteins, an independent evolution without exchange of genetic material between mammalian and avian group A rotavirus strains is likely.
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