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Updated: Jun 25, 2026

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
[An infrared imaging system for detecting electrophoretic mobility shift of DNA-protein complexes]
Yi-fei Wang1, De-hong Cai, Hong Chen
1Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Objective:
To establish a new non-radioactive method for electrophoretic mobility shift assay (EMSA) to investigate the binding between glucocorticoid induced leucine zipper (GILZ) and peroxisome proliferator-activated receptor-gamma 2 (PPARgamma2) promoter oligonucleotides.
Methods:
GILZ protein prepared by prokaryotic expression was linked to PPARgamma2 promoter oligonucleotides end-labeled with IRDye 800 infrared dye. The DNA-protein complex was separated with non-denatured polyacrylamide gel and scanned with the Odyssey. Infrared Imaging System.
Results:
One lane of DNA-protein complex was clearly presented, and the signal intensity increased along with the increment of the protein load.
Conclusion:
This infrared imaging system can be used for EMSA for detecting the DNA-protein complex with high sensitivity efficiency and allows easy operation.
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