Related Experiment Video
Updated: Jun 25, 2026
Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation
Published on: January 12, 2024
Non-invasive method for in vivo detection of chlorophyll precursors
Kim Anker Kristiansen1, Anastassia Khrouchtchova, Anne Stenbaek
1VKR Research Center Pro-Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, DK-1871, Frederiksberg C, Denmark.
Abstract:
Traditionally chlorophyll (Chl) and Chl precursors have been studied in vitro or in leaf tissue at low temperature. These methods are destructive and make it impossible to work with the same individual plant later on. In this paper we present a method for in vivo detection of Chl and its precursors in seedling plants which can be rescued for further studies. Multi-photon microscopy, which we show to be more reliable in vivo than UV-laser microscopy, is used to detect precursors in the biosynthetic pathway leading to Chl. The sensitivity and ability to distinguish different precursors with this system is compared to current methods. Furthermore, we report on optimization of the spectral scanning method with the aim to minimize the excitation light-evoked photo-conversion of the chlorophyll precursors.
More Related Videos
10:20Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
10:08High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Published on: July 6, 2022