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Related Experiment Video

Updated: Jun 25, 2026

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

Intermediary metabolism.

Bart P Braeckman¹, Koen Houthoofd, Jacques R Vanfleteren

¹Biology Department, Ghent University, K.L.Ledeganckstraat 35, B-9000 Ghent, Belgium.

Wormbook : the Online Review of C. Elegans Biology

|February 28, 2009

Summary

Caenorhabditis elegans exhibits adaptable metabolism, shifting pathways during development, aging, and stress. Dauer diapause involves hypometabolism and altered pathways for enhanced survival.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Caenorhabditis elegans possesses orthologs for key eukaryotic intermediary metabolism enzymes.
Metabolic patterns are dynamic, influenced by developmental stage, aging, and environmental stressors.

Purpose of the Study:

To explore metabolic changes in C. elegans during development, aging, and stress response.
To investigate the metabolic shifts during dauer diapause and their impact on survival.

Main Methods:

Comparative analysis of metabolic pathways based on genetic and biochemical data.
Review of existing literature on C. elegans metabolism under various conditions.

Main Results:

Dauer diapause is characterized by hypometabolism, enhanced oxidative stress resistance, and a shift to microaerobic and anaplerotic pathways.

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Repression of citric acid cycle activity and increased reliance on malate dismutation and glyoxylate pathways are observed in dauer larvae.

Metabolic adaptations in dauer larvae promote prolonged survival and share similarities with metabolic changes in long-lived mutants.

Conclusions:

C. elegans exhibits significant metabolic plasticity enabling adaptation to diverse environmental and developmental conditions.
Metabolic reprogramming, particularly during dauer diapause, is crucial for stress resistance and extended lifespan.
Understanding C. elegans metabolism provides insights into fundamental biological processes and aging.