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Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
Published on: December 31, 2009
An experimental method for detecting blood splatter from retractable phlebotomy and intravascular devices
Donna Haiduven1, Shawn Applegarth, Miloni Shroff
1Department of Global Health, College of Public Health, University of South Florida, Tampa, FL 33612-3805, USA. dhaiduve@hsc.usf.edu
Background:
This study was designed to evaluate the safety of retractable intravascular devices in terms of their potential to produce blood splatter. A method for measuring this blood splatter designed by the research team was used to evaluate 3 specific intravascular devices.
Methods:
Scientific filters were positioned around the retraction mechanisms of the devices and weighed with an analytical scale, both before and after activation, in a simulated vein containing mock venous blood. The difference in filter mass was used as the primary unit of analysis to detect blood splatter. In addition, the filters were visually inspected for the presence or absence of blood.
Results:
A paired t-test revealed significant differences in the prefilter and postfilter groups for 2 of the 3 devices tested (P < .0001). In addition, visible blood was detected on 23% to 40% of the scientific filters for 2 of the devices.
Conclusions:
Our findings indicate a potential for bloodborne pathogen exposure with the use of intravascular devices with a retractable mechanism. This experiment may serve as a model in the design and implementation of future sharps device evaluation protocols to validate the threat of bloodborne pathogen exposure.

