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Related Experiment Videos

Mutants defective in chromosome partitioning in E. coli.

S Hiraga1, H Niki, R Imamura

  • 1Department of Molecular Genetics, Kumamoto University Medical School, Japan.

Research in Microbiology
|February 1, 1991
PubMed
Summary

Escherichia coli nucleoids move to cell quarters during division. MukB protein

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Cell Biology

Background:

  • Replicated daughter chromosomes (nucleoids) in Escherichia coli exhibit non-progressive, abrupt movement during cell division.
  • This movement is crucial for positioning nucleoids at future daughter cell centers.
  • Mutations in the muk gene lead to spontaneous production of anucleate cells.

Purpose of the Study:

  • To investigate the role of muk gene products in controlling chromosome positioning during Escherichia coli cell division.
  • To elucidate the structural and functional properties of the MukB protein.

Main Methods:

  • Analysis of experimental results on nucleoid movement in Escherichia coli.
  • Deduction of the MukB protein's amino acid sequence and predicted secondary structure.

Related Experiment Videos

  • Localization of mukC and mukD genes on the chromosome map.

    • Main Results:

    • Muk gene products control chromosome positioning at quarter positions.
    • MukB protein (177-kDa) possesses distinct structural domains, including nucleotide-binding and coiled-coil regions.
    • Predicted structure suggests MukB protein generates force for nucleoid positioning.

    Conclusions:

    • The MukB protein is likely involved in generating the force necessary for nucleoid positioning during cell division.
    • Muk gene products are essential for accurate chromosome segregation in Escherichia coli.
    • MukA is identical to TolC, an outer membrane protein.