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Related Experiment Video

Updated: Jun 25, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Wrap53, a natural p53 antisense transcript required for p53 induction upon DNA damage.

Salah Mahmoudi¹, Sofia Henriksson, Martin Corcoran

¹Department of Oncology-Pathology, Cancer Centrum Karolinska, Karolinska Institutet, SE-17176 Stockholm, Sweden.

|March 3, 2009

Summary

Researchers discovered Wrap53, an antisense transcript regulating p53. Wrap53 controls p53 mRNA and protein levels, impacting DNA damage response and apoptosis, revealing a novel gene regulation mechanism.

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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

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Published on: September 28, 2012

Area of Science:

Molecular Biology
Genomics
Gene Regulation

Background:

Antisense transcription is common in mammalian genomes.
Its role in gene expression regulation is not fully understood.

Purpose of the Study:

To identify and characterize natural antisense transcripts involved in gene regulation.
To elucidate the functional significance of antisense transcription.

Main Methods:

Identification of Wrap53, a natural antisense transcript of p53.
siRNA knockdown and overexpression of Wrap53.
Analysis of p53 mRNA and protein levels.
Investigation of Wrap53/p53 RNA interactions.
Assessment of p53-dependent apoptosis.

Main Results:

Wrap53 directly regulates endogenous p53 mRNA and protein levels.
Wrap53 targets the 5' untranslated region of p53 mRNA.
Wrap53 knockdown decreases p53 levels and suppresses induction upon DNA damage.
Wrap53 overexpression increases p53 levels.
Wrap53/p53 RNA interaction is crucial for p53 regulation.
Wrap53 induction sensitizes cells to p53-dependent apoptosis.

Conclusions:

Wrap53 represents a novel regulatory pathway for controlling p53.
Antisense-mediated RNA interactions offer a general mechanism for gene regulation in human cells.