Related Experiment Video

Updated: Jun 25, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

RNase L downmodulation of the RNA-binding protein, HuR, and cellular growth

W Al-Ahmadi1, L Al-Haj, F A Al-Mohanna

  • 1Program in BioMolecular Research, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.

Oncogene
|March 3, 2009
PubMed

Insights

Overexpressing Ribonuclease L (RNase L) inhibits cell growth and reduces the RNA-binding protein HuR. This suggests RNase L

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Immunology

Background:

  • Ribonuclease L (RNase L) is an intracellular enzyme crucial for innate immunity.
  • RNase L is also investigated as a potential tumor suppressor.

Purpose of the Study:

  • To investigate the role of RNase L in cellular growth and its relationship with the RNA-binding protein HuR.
  • To explore the mechanism by which RNase L influences cell-cycle progression and tumorigenesis.

Main Methods:

  • Overexpression of RNase L in cells.
  • Comparison of RNase L-null mouse fibroblast lines with wild-type cells.
  • Analysis of HuR expression, cellular growth, and HuR mRNA stability.
  • Reporter assays using the HuR 3' untranslated region (UTR).

Main Results:

  • Overexpression of RNase L decreased cellular growth and downmodulated HuR.
  • RNase L's effect on cell growth and HuR was cell-cycle dependent and correlated with its cytoplasmic localization.
  • RNase L-null cells exhibited increased cellular growth and HuR levels, with enhanced HuR mRNA stability.
  • The HuR 3' UTR was sensitive to RNase L activity.

Conclusions:

  • RNase L negatively regulates cellular growth and HuR, a key regulator of cell-cycle progression.
  • The findings provide a potential mechanism for the tumor suppressor function of RNase L.

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...