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Updated: Jun 25, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Published on: August 16, 2012
Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection
Jonas Kühn1, Frédéric Montfort, Tristan Colomb
1Institute of Imaging and Applied Optics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. jonas.kuehn@a3.epfl.ch
Abstract:
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
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