Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antivasospastic Effects of Hydroxyfasudil, a Rho-Kinase Inhibitor, After Subarachnoid Hemorrhage.

Journal of pharmacological sciences·2020
Same author

Pleiotropic effects of the rho-kinase inhibitor fasudil after subarachnoid hemorrhage: a review of preclinical and clinical studies.

Current vascular pharmacology·2014
Same author

Mechanical evaluation of long titanium alloy clip--comparison of cobalt alloy clip.

Neurologia medico-chirurgica·2013
Same author

Ultraweak background scattered light reveals structure of a diffractive element.

Optics letters·2013
Same author

Calculation method for a quadrature phase-shifting interferometer and its applications.

Applied optics·2013
Same author

Selectable-wavelength low-coherence digital holography with chromatic phase shifter.

Optics express·2012

Related Experiment Video

Updated: Jun 25, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Digital holography with a quadrature phase-shifting interferometer.

Tomohiro Kiire1, Suezou Nakadate, Masato Shibuya

  • 1Department of Media Engineering, Graduate School of Engineering, Tokyo Polytechnic University,1583 Iiyama, Atsugi, Kanagawa 243-0297, Japan.

Applied Optics
|March 3, 2009
PubMed
Summary

A new digital holography method uses a quadrature phase-shifting interferometer for high-speed measurements. This technique achieves excellent image quality with fewer devices compared to traditional methods.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Area of Science:

  • Optics and Photonics
  • Digital Imaging
  • Interferometry

Background:

  • Digital holography enables 3D reconstruction of objects.
  • High-speed measurements are crucial for dynamic phenomena.
  • Traditional phase-shifting methods can be complex and require multiple devices.

Purpose of the Study:

  • To present an alternative, high-speed digital holography method.
  • To demonstrate image quality comparable to existing techniques.
  • To reduce the number of imaging devices required.

Main Methods:

  • Utilizing a quadrature phase-shifting interferometer.
  • Acquiring two quadrature phase-shifting fringe patterns per temporal state.
  • Employing a phase calculation method for hologram phase distribution.
  • Reconstructing object images via Fresnel transform and fast Fourier transform (FFT) algorithm.

Main Results:

  • Achieved image quality equivalent to the four-bucket method.
  • Demonstrated the feasibility of high-speed measurement.
  • Confirmed reduced hardware requirements.
  • Verified results through simulations and experiments.

Conclusions:

  • The quadrature phase-shifting interferometer offers an efficient alternative for digital holography.
  • This method provides high-quality imaging with simplified hardware.
  • It is suitable for high-speed, dynamic measurements.