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Cell lines from the soft tick Ornithodoros moubata.

Lesley Bell-Sakyi1, Daniel Růzek, Ernest A Gould

  • 1The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK. L.Sakyi@ed.ac.uk

Experimental & Applied Acarology
|March 3, 2009
PubMed
Summary

Researchers established six new cell lines from Ornithodoros moubata ticks after long-term culture. These tick cell lines support the replication of tick-borne encephalitis virus, aiding in arbovirus research.

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Area of Science:

  • Veterinary Entomology
  • Cell Biology
  • Virology

Background:

  • Primary cell cultures are crucial for tick-borne disease research.
  • Establishing continuous tick cell lines is challenging but vital for studying tick biology and pathogens.

Purpose of the Study:

  • To establish and characterize new cell lines from the soft tick Ornithodoros moubata.
  • To assess the susceptibility of these cell lines to tick-borne encephalitis virus.

Main Methods:

  • Initiation of primary cell cultures from embryonic and neonatal Ornithodoros moubata.
  • Long-term in vitro maintenance and subculturing of cell lines at 28°C.
  • Serial passaging of tick-borne encephalitis virus in established cell lines.

Main Results:

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  • Six Ornithodoros moubata embryonic/neonatal cell lines (OME/CTVM21-27) were successfully established after 20-25 months.
  • Cells exhibited heterogeneous populations with specific morphological features and formed multicellular structures.
  • Tick-borne encephalitis virus replicated efficiently in OME/CTVM21 cells over ten passages without altering its biological properties.

Conclusions:

  • The established Ornithodoros moubata cell lines provide a valuable tool for tick and arbovirus research.
  • These cell lines support sustained virus replication, demonstrating their utility in virological studies.
  • Further research can utilize these cell lines for investigating tick-borne encephalitis virus and other tick-borne pathogens.