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Updated: Jun 25, 2026
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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Generation and selection of rabbit antibody libraries by phage display
1Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2009
Summary
Rabbit monoclonal antibodies (mAbs) offer high specificity and affinity, providing an infinite supply unlike traditional polyclonal antibodies (pAbs). Phage-display technology enables selection of rabbit mAbs that target mouse and human antigens effectively.
Area of Science:
- Immunology
- Biotechnology
Background:
- Rabbit polyclonal antibodies (pAbs) have been used for decades.
- Rabbit monoclonal antibodies (mAbs) are gaining interest due to their defined nature and infinite supply.
- Rabbit antibodies offer high specificity and avidity (pAbs) or affinity (mAbs).
Purpose of the Study:
- To highlight the advantages of rabbit monoclonal antibodies (mAbs).
- To introduce phage-display technology for selecting rabbit mAbs.
- To demonstrate the selection of rabbit mAbs with cross-reactivity to mouse and human antigens.
Main Methods:
- Utilizing phage-display technology.
- Employing chimeric rabbit/human Fab libraries.
- Selecting for high-affinity and specific rabbit mAbs.
Main Results:
- Rabbit mAbs can be selected with high affinity and specificity.
- Selected rabbit mAbs demonstrate cross-reactivity with both mouse and human antigens.
- Phage-display facilitates the development of rabbit mAbs.
Conclusions:
- Rabbit mAbs are valuable reagents with significant potential.
- Phage-display technology is effective for generating rabbit mAbs.
- Rabbit mAbs can be engineered for broad antigen recognition.
