Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

236.9K
Overview
236.9K
Real Time RT-PCR02:57

Real Time RT-PCR

64.6K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prospective Double-Blind Randomized Study of Single Dose Bone Marrow-Derived Mesenchymal Stromal Cells versus Placebo for Steroid-Refractory Acute Graft-Versus-Host Disease.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie·2025
Same author

The Molecular Diagnosis of Invasive Fungal Diseases with a Focus on PCR.

Diagnostics (Basel, Switzerland)·2025
Same author

Pilot Study Comparing the In Vitro Response of Circulating Monocytes to <i>Aspergillus fumigatus</i> Swollen Conidia in Patients with Chronic Graft-Versus-Host Disease and Healthy Volunteers.

Journal of fungi (Basel, Switzerland)·2025
Same author

A longitudinal study assessing Aspergillus polymerase chain reaction (PCR) performance through participation in external quality assessment schemes from 2019 to 2022.

Medical mycology·2025
Same author

The diagnosis of mucormycosis by PCR in patients at risk: a systematic review and meta-analysis.

EClinicalMedicine·2025
Same author

Multicentric prospective evaluation of Pneumocystis jirovecii fungal load in bronchoalveolar lavage fluid fractions using qPCR.

Medical mycology·2025

Related Experiment Video

Updated: Jan 14, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

6.8K

Aspergillus PCR formidable challenges and progress.

Lena Klingspor1, Juergen Loeffler

  • 1Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden. lena.klingspor@ki.se

Medical Mycology
|March 3, 2009
PubMed
Summary
This summary is machine-generated.

Rapid polymerase chain reaction (PCR) tests offer a promising solution for early detection of invasive aspergillosis (IA) in immunocompromised patients. This molecular method shows high potential for sensitive and specific diagnosis, improving patient outcomes.

More Related Videos

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

11.0K
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
08:01

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

28.4K

Related Experiment Videos

Last Updated: Jan 14, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

6.8K
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

11.0K
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
08:01

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

28.4K

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Invasive aspergillosis (IA) is a severe infection with high mortality (80-90%) in immunocompromised individuals, particularly stem cell transplant recipients.
  • Conventional diagnostic methods like blood cultures lack utility for Aspergillus fungemia, and older non-culture techniques have insufficient sensitivity and specificity.
  • Early diagnosis and treatment are crucial for improving outcomes in IA patients, necessitating advanced diagnostic tools.

Purpose of the Study:

  • To evaluate the clinical applicability of a newly established diagnostic assay combining manual and automated DNA extraction with real-time PCR for detecting Aspergillus DNA.
  • To present data on Aspergillus real-time PCR (R-T PCR) results in immunocompromised patients suspected of invasive fungal infections.
  • To highlight the need for standardized molecular assays and address ongoing questions regarding their use in clinical practice.

Main Methods:

  • Development and implementation of a diagnostic assay utilizing manual and robotic extraction for Candida and Aspergillus DNA.
  • Application of real-time PCR (R-T PCR) for the detection of fungal DNA in patient samples.
  • Analysis of a large number of samples from patients with suspected invasive fungal infections.

Main Results:

  • Real-time PCR assays demonstrate high potential for sensitivity and specificity in detecting Aspergillus DNA.
  • Various PCR assays targeting different gene regions and detection methods have been developed, showing variability in feasibility.
  • Data on Aspergillus R-T PCR results in immunocompromised patients will be presented, focusing on clinical applicability.

Conclusions:

  • Real-time PCR offers a promising rapid diagnostic method for invasive aspergillosis, addressing limitations of conventional tests.
  • Standardization of PCR assays is critical, with initiatives like the European Aspergillus PCR Initiative (EAPCRI) working towards European standards.
  • Further prospective studies are needed to evaluate the benefits of early antifungal therapy guided by R-T PCR results in high-risk patients.