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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

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Related Experiment Video

Updated: Jun 25, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

[Peptide TAT modified polyethylenimine-beta-cyclodextrin for gene delivery].

Li-Hua Lai1, Qi-Ying Jiang, Dan Chen

  • 1Institute of Immunology, College of Medicine, Zhejiang University, Hangzhou 310058, China.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|March 3, 2009
PubMed
Summary

A novel gene delivery vector, TAT-PEI-beta-CyD, was successfully developed. This new vector demonstrates reduced cytotoxicity and enhanced gene transfection efficiency compared to traditional PEI 25 kDa.

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Last Updated: Jun 25, 2026

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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Published on: September 2, 2015

Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Context:

  • Gene delivery vectors are crucial for therapeutic applications.
  • Polyethylenimine (PEI) is a common gene carrier but has limitations.
  • Novel vectors are needed to improve safety and efficacy.

Purpose:

  • To synthesize and characterize a novel gene delivery vector, TAT-PEI-beta-CyD.
  • To evaluate the physicochemical properties, cytotoxicity, and transfection efficiency of the new vector.

Summary:

  • TAT peptide was conjugated to PEI-beta-cyclodextrin (beta-CyD) to create TAT-PEI-beta-CyD.
  • The vector efficiently condensed DNA into nanoparticles around 100 nm.
  • In vitro studies showed lower cytotoxicity and higher transfection efficiency than PEI 25 kDa.

Impact:

  • The developed TAT-PEI-beta-CyD vector shows promise for improved gene therapy applications.
  • This novel vector offers a safer and more effective alternative for gene delivery.
  • Further research can explore its potential in various therapeutic contexts.