Related Experiment Video
Updated: Jun 25, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
[Chiral separation of bisoprolol using high performance liquid chromatography with amylose chiral stationary phase]
Fang Li1, Jiayang Li, Huayan Zhang
1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. lifang198467@163.com
Abstract:
A high performance liquid chromatographic (HPLC) method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed. Tris (3,5-dimethylphenyl carbamate) amylase chiral stationary phase was used in the normal-phase mode. The effects of the concentration of organic solvents (iso-propanol and ethanol), flow rate and column temperature on the enantiomer separation were studied systematically. The best separation was obtained with hexane-ethanol-diethyl amine (88 : 12 : 0.1, v/v/v) as mobile phase at 20 degrees C. The optimal flow rate was 0.6 mL/min. The detection wavelength was set at 270 nm. By using this method, the enantiomers of bisoprolol can be separated quickly and easily.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Affinity Chromatography
