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[The effect of ischemic postconditioning on apoptosis induced by acute hot renal ischemic-reperfusion]
Yu-chun Zhu1, Tie-long Tang, Shu Cui
1Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effects of ischemic postconditioning(IPo) on cellular apoptosis in acute renal ischemic-reperfusion injury (IRI) in rats.
Methods:
Model of acute renal ischemic-reperfusion injury (IRI) was constructed using Wistar rats. Forty healthy male rats were divided into 4 groups randomly,including group C,group IRI, group IPo and group Ado. Rats of group C received right nephronectomy and separation of left renal vessels. Before reperfusion, group IPo was perfused for 10 s, following ischemic reperfusion for another 10 s with 6 cycles. Group Ado was infused with adenosine intravenously 10 min before ischemia. Cellular apoptosis was determined with TUNEL (TdT-mediated dUTP Nick End Labelling), flowcytometry and histopathological staining.
Results:
Compared with group C, the apoptotic index increased predominatly in group IRI (P<0.05), whereas, apoptosis index decreased obviousely in group IPo, Ado (P<0.05). In addition, the apoptosis index between group IPo and Ado was not statistically different.
Conclusion:
Ischemic postconditioning could decrease cellular apoptosis induced by IRI in rat kidney.
