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Updated: Jun 25, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
[Impact of human isophane insulin on the evaluation of beta cell functions]
Mei Zhang1, Lin Zhang, Ming-jie Huang
1Division of Hormone Assay, Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To evaluate the beta cell functions with and without human Isophane insulin treatments.
Methods:
Thirty patients with type 2 diabetes mellitus who followed the human Isophane insulin therapeutic regimen at bedtime were given 100 g steamed bread tests in two consecutive days. Then the human Isophane insulin treatments were stopped and the 100 g steamed bread tests were repeated in the next day. The fasting and 1, 2, 3 h after meal plasma glucose, serum insulin, C-peptide and proinsulin were measured.
Results:
(1) The fasting and 1,2,3 h after meal plasma glucose increased while the serum insulin decreased after the human Isophane insulin treatments were stopped (P<0.05). (2) The fasting and 1, 2 h after meal C peptide increased (P<0.05) after the human Isophane insulin treatments were stopped. (3) The fasting and 1, 2, 3 h after meal proinsulin increased after the human isophane insulin treatments were stopped (P<0.05). (4) The area under the insulin curve decreased, while, the area under the C peptide increased after the human Isophane insulin treatments were stopped (P<0.05).
Conclusion:
Human Isophane insulin treatments should not be stopped to evaluate the beta cell functions of the patients who are undergoing Isophane insulin treatment.
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