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Mouse In Vivo Placental Targeted CRISPR Manipulation
Published on: April 14, 2023
[SEREX screening of human placenta antigens]
Qing-yuan Jiang1, Qing Xiong, Xia Zhao
1State Key Laboratory of Biotherapy, West China Hosptial, Sichuan University, Chengdu 610041, China.
Summary
This study identified key human placenta antigens using a modified SEREX technique. These findings offer insights into embryo development and potential diagnostic applications.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Context:
- The human placenta plays a crucial role in fetal development and maternal-fetal interactions.
- Identifying specific placental antigens is vital for understanding pregnancy physiology and pathology.
Purpose:
- To screen and isolate potential human placenta antigens using a modified serological analysis of recombinant cDNA expression library (SEREX) technique.
- To analyze the identified antigens for their role in embryo development.
Summary:
- A modified xenogeneic immune SEREX approach was employed, utilizing rabbit immune sera against human chorionic tissue to screen a placenta cDNA expression library.
- Two rounds of screening yielded 69 positive clones from 12 distinct genes, with chorionic somatomammotropin hormone 1 and 2 (CSH1 and CSH2) being the most abundant (82.6%).
- The identified antigens, particularly CSH1 and CSH2, are implicated as important in placental function and embryo development.
Impact:
- This study demonstrates the efficacy of modified SEREX for isolating human placenta antigens.
- The identified antigens may serve as biomarkers for pregnancy-related conditions and contribute to a deeper understanding of embryonic development.
