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Related Experiment Videos

Triiodothyronine receptors during maturation.

L J DeGroot, M Robertson, P A Rue

    Endocrinology
    |June 1, 1977
    PubMed
    Summary
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    Rat liver nuclear triiodothyronine (T3) binding protein (NTBP) capacity and affinity increase significantly during maturation. However, T3 receptor occupancy decreases in older rats, indicating complex thyroid hormone regulation.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Developmental Biology

    Background:

    • Thyroid hormones, particularly triiodothyronine (T3), are crucial for mammalian development and metabolism.
    • Nuclear triiodothyronine binding protein (NTBP) plays a key role in mediating T3's effects.
    • Understanding the developmental changes in NTBP is essential for comprehending thyroid hormone action.

    Purpose of the Study:

    • To investigate the developmental changes in the capacity and affinity of rat liver NTBP for T3.
    • To correlate these changes with T3 content and receptor occupancy.
    • To examine the impact of certain hormones on NTBP characteristics.

    Main Methods:

    • Quantification of T3 binding capacity and affinity (Ka) in rat liver nuclei at different ages.

    Related Experiment Videos

  • Measurement of nuclear T3 content and the proportion of occupied receptors.
  • Assessment of mitochondrial alpha-glycerophosphate dehydrogenase activity.
  • Hormonal administration studies in immature female rats.

    • Main Results:

    • Liver NTBP capacity for T3 quadrupled from birth to 50-120 days, independent of DNA or tissue weight.
    • T3 binding affinity (Ka) doubled during this maturation period.
    • Nuclear T3 content paralleled NTBP capacity, while receptor occupancy decreased with age.
    • Mitochondrial alpha-glycerophosphate dehydrogenase activity showed less change compared to NTBP.
    • Estrogen, testosterone, and dexamethasone did not affect NTBP capacity or affinity in immature rats.

    Conclusions:

    • Rat liver NTBP undergoes substantial developmental increases in capacity and affinity, suggesting enhanced T3 responsiveness during maturation.
    • The decrease in receptor occupancy with age indicates a complex regulatory mechanism for thyroid hormone action.
    • Exogenous hormones tested did not influence the fundamental properties of liver NTBP during development.