Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

Multi-round virus filter integrity test sensitivity.

Christopher J Dowd1

  • 1Genentech, Inc., South San Francisco, California 94080, USA. cjdowd@gene.com

Biotechnology and Bioengineering
|March 4, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Binding inhibition of angiogenic factors by heparan sulfate proteoglycans in aqueous humor: potential mechanism for maintenance of an avascular environment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2003
Same journal

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species.

Biotechnology and bioengineering·2026
Same journal

Artificial Intelligence-Powered Algal Biodiesel: The Future of Biofuel Production Through Data-Driven Biotechnology.

Biotechnology and bioengineering·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
See all related articles

Integrity testing of virus filters in biopharmaceutical processes is crucial. This study shows the forward flow diffusion test

Area of Science:

  • Biopharmaceutical Manufacturing
  • Filtration Technology
  • Process Validation

Background:

  • Virus filtration is a key step in biopharmaceutical recovery, essential for ensuring product safety.
  • Increasing production scales necessitate the use of multiple virus filter elements in parallel.
  • Effective integrity testing is vital for validating the performance of these filter assemblies.

Purpose of the Study:

  • To evaluate the sensitivity of the forward flow diffusion test for single and multiple virus filters in a housing.
  • To develop a method for estimating undetected flaws in large virus filter assemblies.
  • To determine the impact of filter assembly size on integrity test sensitivity.

Main Methods:

  • Developed an approach to estimate the largest undetectable hole size in a single filter within a larger assembly.

More Related Videos

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

Related Experiment Videos

Last Updated: Jun 25, 2026

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

  • Calculated the integrity test limited minimum log reduction value (LRV) based on hole size and filter number.
  • Analyzed the sensitivity of the LRV to the number of filters per housing.

    • Main Results:

    • The minimum LRV, indicating the smallest detectable flaw, is largely insensitive to the number of filters in a housing.
    • The analysis suggests minimal benefit in using individual housings for each filter.
    • The likelihood of significant filter damage is considered very low.

    Conclusions:

    • Current integrity testing methods remain effective even with multiple virus filters in a single housing.
    • Adjusting test specifications for larger filter assemblies is likely unnecessary.
    • This simplifies the validation and integrity testing strategy for large-scale biopharmaceutical manufacturing.