Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inactivation of PETase at Interfaces Inhibits PET Plastic Depolymerization.

ACS sustainable chemistry & engineering·2026
Same author

Gradual Morphological Tuning in Polymer Microspheres via Pickering Emulsion Synthesis: Architecture-Controlled Dye Adsorption and Encapsulation.

International journal of molecular sciences·2026
Same author

Morphological Design and Synthesis of Nanoparticles (Second Edition).

Nanomaterials (Basel, Switzerland)·2026
Same author

Electrokinetic nanoparticle transport in an interconnected porous environment: Decoupling cavity escape and directional bias.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Mixed lipid bilayers enable enhanced stability and activity retention of Lipase A in low-pH environments.

Colloids and surfaces. B, Biointerfaces·2025
Same author

Thermostable Enzyme Variants in the Lower Mevalonate Pathway Improve Isoprenoid Production by Cell-Free Biocatalysis.

ACS sustainable chemistry & engineering·2025

Related Experiment Video

Updated: Jun 25, 2026

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
13:15

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Published on: July 18, 2014

Hydrophobic interaction microscopy: mapping the solid/ liquid interface using amphiphilic probe molecules.

Andrei Honciuc1, Denver Jn Baptiste, Daniel K Schwartz

  • 1Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|March 4, 2009
PubMed
Summary

This study shows how fluorescent molecules can map tiny changes in surface hydrophobicity. This technique offers a more sensitive and reliable way to visualize hydrophobic patterns on surfaces.

More Related Videos

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

Related Experiment Videos

Last Updated: Jun 25, 2026

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
13:15

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Published on: July 18, 2014

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

Area of Science:

  • Surface science
  • Physical chemistry
  • Materials science

Background:

  • Amphiphilic molecule behavior is sensitive to interfacial chemical properties.
  • Mapping surface hydrophobicity is crucial for understanding interfacial phenomena.

Purpose of the Study:

  • To demonstrate that amphiphilic molecule behavior can map subtle spatial variations in surface hydrophobicity.
  • To compare fluorescence microscopy with lateral force microscopy for surface characterization.

Main Methods:

  • Utilized total internal reflection fluorescence microscopy (TIRFM) to observe single fluorescently labeled fatty acid molecules.
  • Created patterned surfaces by photodegrading trimethylsilane-modified fused silica.
  • Varied hydrophobic contrast by controlling ultraviolet radiation dose.

Main Results:

  • Cumulative fluorescence images revealed structural features consistent with photopatterned surfaces.
  • Fluorescence contrast systematically correlated with hydrophobic contrast.
  • Fluorescent probes provided more sensitive, reproducible, and reliable imaging of lateral hydrophobic variations compared to lateral force microscopy.

Conclusions:

  • Single-molecule fluorescence microscopy is a powerful tool for mapping surface hydrophobicity.
  • This method offers enhanced sensitivity and reliability for characterizing patterned hydrophobic surfaces.