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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Published on: October 9, 2016
The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation.
Ryuta Muromoto1, Naohisa Taira, Osamu Ikeda
1Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo 060-0812, Japan.
Biochemical and Biophysical Research Communications
|March 4, 2009
Summary
MAGOH protein inhibits the interaction between Signal transducer and activator of transcription 3 (STAT3) and Y14. Reducing MAGOH enhances STAT3 transcriptional activity, revealing MAGOH
Area of Science:
- Molecular Biology
- Cell Biology
- Gene Regulation
Background:
- Signal transducer and activator of transcription 3 (STAT3) is crucial for physiological processes, activated by cytokines and growth factors.
- Y14, an exon-junction complex (EJC) component, was previously shown to bind STAT3 and enhance its transcriptional activity by affecting DNA binding.
- STAT3's role in gene regulation is complex and influenced by protein interactions.
Purpose of the Study:
- To investigate the endogenous interaction between STAT3 and Y14.
- To determine the role of MAGOH, a Y14 partner, in regulating the STAT3-Y14 interaction and STAT3 transcriptional activity.
Main Methods:
- Co-immunoprecipitation assays to confirm endogenous STAT3-Y14 interaction.
- Assessment of MAGOH's effect on STAT3-Y14 complex formation.
- Small-interfering RNA (siRNA) to reduce MAGOH expression and measure its impact on interleukin-6-induced gene expression.
Main Results:
- STAT3 and Y14 were demonstrated to interact endogenously.
- MAGOH was found to inhibit the formation of the STAT3-Y14 complex.
- Reduction of MAGOH expression using siRNA led to enhanced interleukin-6-induced gene expression, indicating increased STAT3 activity.
Conclusions:
- MAGOH negatively regulates the transcriptional activation of STAT3.
- MAGOH achieves this regulation by interfering with the complex formation between STAT3 and Y14.
- This study elucidates a novel regulatory mechanism for STAT3 activity involving the exon-junction complex protein MAGOH.
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