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Updated: Jun 25, 2026

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
Published on: April 23, 2021
Crystallization and preliminary X-ray diffraction analysis of a putative two-domain-type laccase from a metagenome
Hirofumi Komori1, Kentaro Miyazaki, Yoshiki Higuchi
1Department of Life Science, University of Hyogo, Koto, Kamigori-cho, Ako-gun, Japan.
Abstract:
A putative two-domain-type laccase retrieved from a metagenome was successfully crystallized using the sitting-drop vapour-diffusion method. Data were collected to a resolution of 1.7 A at 100 K using synchrotron radiation. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.67, b = 100.95, c = 124.11 A. The self-rotation function showed the presence of a noncrystallographic threefold axis in the structure. The presence of one trimer in the asymmetric unit yielded a Matthews coefficient (V(M)) of 2.05 A(3) Da(-1) and a solvent content of 40%.
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