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Linkage disequilibrium between four MTTP gene polymorphisms in a Mexican population
K E Luévano1, J R González, F J Perea
1División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
Annals of Human Biology
|March 4, 2009
Summary
Genetic variations in the microsomal triglyceride transfer protein (MTTP) gene were analyzed in a Mexican population. Specific MTTP polymorphisms were identified as potentially useful for future association studies.
Area of Science:
- Genetics and Molecular Biology
- Cardiovascular Disease Research
- Metabolic Disease Research
Background:
- The microsomal triglyceride transfer protein (MTTP) is crucial for lipoprotein metabolism.
- MTTP gene polymorphisms are linked to coronary heart disease and diabetes risk.
- These diseases are leading causes of death in Mexico.
Purpose of the Study:
- To determine allele, genotype, and haplotype frequencies of six MTTP gene polymorphisms.
- To analyze these frequencies in an unselected Mexican population.
Main Methods:
- DNA sequencing of polymerase chain reaction products from 155 Mexican individuals.
- Evaluation of Hardy-Weinberg equilibrium, genetic variability, linkage disequilibrium, and neutrality tests.
Main Results:
- Rare alleles for all six polymorphisms exceeded 1% frequency.
- Genotype distributions adhered to Hardy-Weinberg equilibrium.
- Significant linkage disequilibrium was observed among promoter and I/T 128 polymorphisms.
- Twelve haplotypes were identified, with GATGGT and TTCGGC being most common.
- Population diversity patterns deviated from the standard neutral model.
Conclusions:
- The -493 G/T, -400 A/T, -164 T/C, and I/T 128 MTTP polymorphisms are valuable for association studies in the Mexican population.
- These findings contribute to understanding genetic predispositions to cardiovascular and metabolic diseases in this demographic.
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