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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Published on: February 27, 2020

Post analysis data acquisition for the iterative MS/MS sampling of proteomics mixtures.

Michael R Hoopmann1, Gennifer E Merrihew, Priska D von Haller

  • 1University of Washington, Department of Genome Sciences, Seattle, Washington 98195, USA.

Journal of Proteome Research
|March 5, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces an automated method to improve peptide identification in mass spectrometry. The new approach enhances the detection of missed peptides, increasing protein identification in complex mixtures.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Microcapillary liquid chromatography-tandem mass spectrometry (microLC-MS/MS) is a standard technique for peptide identification.
  • Limitations in MS/MS sampling speed hinder the identification of all detectable peptides, even with advanced instruments.

Purpose of the Study:

  • To develop an automated approach for enhancing peptide and protein identification in complex mixtures.
  • To overcome the limitations of MS/MS sampling speed in proteomics experiments.

Main Methods:

  • Utilizing high-resolution microLC-MS data to identify molecular species in a mixture.
  • Developing an automated system to direct MS/MS acquisition towards previously missed precursor ions.
  • Implementing a unique workflow with custom software for data analysis.

Main Results:

  • Increased coverage of molecular species sampled by MS/MS.
  • A greater number of peptides and proteins identified in technical and biological replicates.
  • Improved identification of molecular features in complex protein mixtures.

Conclusions:

  • The developed automated approach significantly enhances peptide and protein identification in proteomics.
  • This method offers a valuable tool for deeper analysis of complex biological samples using microLC-MS/MS.