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Related Experiment Video

Updated: Jun 25, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
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Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Two-photon imaging using adaptive phase compensated ultrashort laser pulses.

Peng Xi1, Yair Andegeko, Dmitry Pestov

  • 1Shanghai Jiao Tong University, Department of Biomedical Engineering, Institute for Laser Medicine and Biophotonics, Shanghai 200240 China.

Journal of Biomedical Optics
|March 5, 2009
PubMed
Summary

Multiphoton intrapulse interference phase scanning (MIIPS) adaptive optics corrected phase distortions in a two-photon microscope. This resulted in significantly improved imaging signals for cells, tissues, and biological specimens.

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Area of Science:

  • Optics and Photonics
  • Microscopy
  • Biomedical Engineering

Background:

  • High-numerical-aperture objectives and microscope optics introduce high-order phase distortions.
  • These distortions limit the effectiveness of nonlinear microscopy techniques like two-photon excitation and second harmonic generation.
  • Accurate pulse shaping is crucial for optimizing ultrashort pulse delivery in microscopy.

Purpose of the Study:

  • To measure and compensate for high-order phase distortions in a two-photon laser-scanning microscope.
  • To enhance the signal strength of two-photon and second harmonic generation imaging.
  • To demonstrate the utility of adaptive pulse shaping for biological imaging applications.

Main Methods:

  • Utilized an adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS).

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Direct Imaging of Laser-driven Ultrafast Molecular Rotation
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Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

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Last Updated: Jun 25, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

  • Employed a prism-pair compressor to manage pulse dispersion.
  • Confirmed sub-12-fs pulse delivery using interferometric autocorrelation measurements at the focal plane.

    • Main Results:

    • Achieved broad-bandwidth (approx. 100 nm) ultrashort pulse delivery to the focal plane.
    • Observed a 6-to-11-fold improvement in two-photon excitation fluorescence signal in cells and tissue.
    • Demonstrated up to a 19-fold improvement in second harmonic generation signal from rat tendon.

    Conclusions:

    • MIIPS-controlled adaptive optics effectively cancel high-order phase distortions in two-photon microscopy.
    • Optimized pulse shaping significantly enhances nonlinear imaging signal strength.
    • This technique offers substantial improvements for imaging biological samples and tissues.