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A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
Published on: October 1, 2017
Nonmonotonic density dependence of the diffusion of DNA fragments in low-salt suspensions
Mathieu G McPhie1, Gerhard Nägele
1Institut für Festkörperforschung, Forschungszentrum Jülich, D-52425 Jülich, Germany.
Abstract:
The high linear charge density of 20-base-pair oligomers of DNA is shown to lead to a striking nonmonotonic dependence of the long-time self-diffusion on the concentration of DNA in low-salt conditions. This generic nonmonotonic behavior results from the strong coupling between the electrostatic and solvent-mediated hydrodynamic interactions, from the renormalization of these electrostatic interactions at large separations, and specifically from the dominance of the far-field hydrodynamic interactions caused by the strong repulsion between the DNA fragments.
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